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Proceedings Paper

Two-photon excited imaging of photosensitizers in tissues
Author(s): Mariah L. Coleno; Vincent P. Wallace; Chung-Ho Sun; Andrew K. Dunn; Michael W. Berns; Bruce J. Tromberg
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Paper Abstract

Two-photon microscopy (TPM) is a non-invasive biological imaging technique that can be used to selectively image cellular activity and photosensitizer (PS) localization within highly scattering epithelial tissues at depths of approximately 200 micrometer with submicron resolution. The principal objective of this study was to develop a model system for understanding the impact of photodynamic therapy on cellular and extracellular matrix remodeling in biological tissues. An artificial tissue model (RAFT) composed of collagen, embedded fibroblasts, and macrophage cells has been developed for this purpose. TPM is utilized to monitor extracellular matrix remodeling following PDT by imaging collagen/elastin autofluorescence. Selective uptake of photosensitizers by specific cellular components in the matrix can also be visualized by TPM.

Paper Details

Date Published: 1 June 1999
PDF: 7 pages
Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); doi: 10.1117/12.349217
Show Author Affiliations
Mariah L. Coleno, Beckman Laser Institute and Medical Clinic and Univ. of California/Irvine (United States)
Vincent P. Wallace, Beckman Laser Institute and Medical Clinic (United Kingdom)
Chung-Ho Sun, Beckman Laser Institute and Medical Clinic (United States)
Andrew K. Dunn, Beckman Laser Institute and Medical Clinic (United States)
Michael W. Berns, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic and Univ. of California/Irvine (United States)


Published in SPIE Proceedings Vol. 3604:
Optical Diagnostics of Living Cells II
Daniel L. Farkas; Robert C. Leif; Bruce J. Tromberg, Editor(s)

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