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Proceedings Paper

Intracellular gradients of free calcium visualized in sensory and neuronal cells by a high-performance fluorescence imaging system
Author(s): Alberto Colavita; Gabriele Capello; Razaq B. Ijaduola; Antonio Cunei; Laura Lagostena; Marco Canepari; Fabio Mammano
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Paper Abstract

This paper describes results obtained with a novel imaging system based on a fast CCD device. Sensor's output was digitized at 12 bit/pixel by customized electronics, reaching acquisition rates as high as 800 frames/s with a full-frame resolution of 128 X 128 pixels. The software developed for the project permitted the sequential capture of thousands of images directly to host PC RAM without frame loss even at the maximum readout rate (16 MHz). It is shown that the high spatio-temporal resolution of this apparatus is of value when investigating the time-course of rapid intracellular Ca2+ fluorescence transients, particularly those associated with neuronal action potentials near physiological temperature.

Paper Details

Date Published: 1 June 1999
PDF: 7 pages
Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); doi: 10.1117/12.349212
Show Author Affiliations
Alberto Colavita, INFN/Abdus Salam International Ctr. for Theoretical Physics (Italy)
Gabriele Capello, INFN/Abdus Salam International Ctr. for Theoretical Physics (Italy)
Razaq B. Ijaduola, INFN/Abdus Salam International Ctr. for Theoretical Physics (Italy)
Antonio Cunei, INFN/Abdus Salam International Ctr. for Theoretical Physics (Italy)
Laura Lagostena, INFM/International School for Advanced Studies (Italy)
Marco Canepari, INFM/International School for Advanced Studies (Italy)
Fabio Mammano, INFM/International School for Advanced Studies (Italy)


Published in SPIE Proceedings Vol. 3604:
Optical Diagnostics of Living Cells II
Daniel L. Farkas; Robert C. Leif; Bruce J. Tromberg, Editor(s)

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