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Proceedings Paper

Addition of a second lanthanide ion to increase the luminescence of europium(III) macrocyclic complexes
Author(s): Alfred J. Bromm; Robert C. Leif; John R. Quagliano; Lidia M. Vallarino
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Paper Abstract

At present, the microscopic visualization of luminescent labels containing lanthanide(III) ions, primarily europium(III), as light-emitting centers is best performed with time-gated instrumentation, which by virtually eliminating the background fluorescence results in an improved signal to noise ratio. However, the use of the europium(III) macrocycle, Quantum DyeTM, in conjunction with the strong luminescence enhancing effect (cofluorescence) of yttrium(III) or gadolinium(III), can eliminate the need for such specialized instrumentation. In the presence of Gd(III), the luminescence of the Eu-macrocycles can be conveniently observed with conventional fluorescence instrumentation at previously unattainable low levels. The Eu(III) 5DO yields 7F2 emission of the Eu-macrocycles was observed as an extremely sharp band with a maximum at 619 nm and a clearly resolved characteristic pattern. At very low Eu- macrocycle concentrations, another sharp emission was detected at 614 nm, arising from traces of Eu(III) present in even the purest commercially available gadolinium products. Discrimination of the resolved emissions of the Eu-macrocycle and Eu(III) contaminant should provide a means to further lower the limit of detection of the Eu-macrocycle.

Paper Details

Date Published: 1 June 1999
PDF: 10 pages
Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); doi: 10.1117/12.349209
Show Author Affiliations
Alfred J. Bromm, Virginia Commonwealth Univ. (United States)
Robert C. Leif, Newport Instruments (United States)
John R. Quagliano, Los Alamos National Lab. (United States)
Lidia M. Vallarino, Virginia Commonwealth Univ. (United States)


Published in SPIE Proceedings Vol. 3604:
Optical Diagnostics of Living Cells II
Daniel L. Farkas; Robert C. Leif; Bruce J. Tromberg, Editor(s)

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