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Proceedings Paper

Basic mechanisms of formation of integral scattering indicatrix of optically soft spherical biological particles
Author(s): Nickolay V. Shepelevich; Inna V. Prostakova; Valeriy N. Lopatin
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Paper Abstract

The work is devoted to the theoretical investigation of the formation of a radiation scattered by optically soft spherical biological particles into the solid angle (Theta) o (integral indicatrix). Integral light-scattering indicatrix F in the range of the phase-shift parameter (Delta) from 0.01 to 80 and of the relative refractive index m (m equals n + i(chi )): n less than or equal to 1.2; (chi) less than or equal to 10-2 has been studied. Fraunhofer diffraction, geometric optics, Wentzel-Kramers-Brillouin approximation, the Mie theory have been applied for the analyses of the light- scattering indicatrix. The theoretical and numerical investigation reveals that the integral light-scattering indicatrix can be represented as a combination of two mechanisms, namely, Fraunhofer diffraction and geometrical optics ((Delta) greater than or equal to 3). The feasibility of determining the relative refractive index of the studied biological particles has been shown.

Paper Details

Date Published: 17 May 1999
PDF: 8 pages
Proc. SPIE 3599, Optical Diagnostics of Biological Fluids IV, (17 May 1999); doi: 10.1117/12.348379
Show Author Affiliations
Nickolay V. Shepelevich, Institute of Computer Modeling (Russia)
Inna V. Prostakova, Institute of Computer Modeling (Russia)
Valeriy N. Lopatin, Institute of Computer Modeling (Russia)


Published in SPIE Proceedings Vol. 3599:
Optical Diagnostics of Biological Fluids IV
Alexander V. Priezzhev; Toshimitsu Asakura, Editor(s)

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