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Proceedings Paper

Modeling aberrations due to mismatched layers for 3-D microscopy
Author(s): Sarah F. Gibson; Frederick Lanni
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Paper Abstract

Oil immersion microscope objective lenses have been designed and optimized to study thin, two-dimensional sections of objects that are monuted in a medium which is index-matched to the immersion oil and located immediately below the coverslip. The 3-D image formed by an objective lens will be subject to aberrations if any of the design conditions are not met. The degree of the aberrations can be calculated by comparing the optical paths of rays traveling through the microscope in a non-design system to those of correspondig rays traveling through the microscope under the design conditions.

Paper Details

Date Published: 1 July 1990
PDF: 2 pages
Proc. SPIE 1319, Optics in Complex Systems, (1 July 1990); doi: 10.1117/12.34810
Show Author Affiliations
Sarah F. Gibson, Carnegie Mellon Univ. (United States)
Frederick Lanni, Carnegie Mellon Univ. (United States)

Published in SPIE Proceedings Vol. 1319:
Optics in Complex Systems
F. Lanzl; H.-J. Preuss; G. Weigelt, Editor(s)

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