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Proceedings Paper

Real-time confocal two-photon fluorescence microscope using a rotating microlens array
Author(s): Katsumasa Fujita; Tomoyuki Kaneko; Osamu Nakamura; Masahito Oyamada; Tetsuro Takamatsu; Satoshi Kawata
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Paper Abstract

The use of a confocal pinhole-array in a real-time two-photon fluorescence microscope with a microlens array is proposed to increase the three-dimensional resolution and the depth- penetration property. Enhancement of the lateral and axial resolution in the proposed microscope is observed in our experiments. Comparisons of images taken with and without the pinhole-array to verify the sharp depth-imaging performance are shown.

Paper Details

Date Published: 7 May 1999
PDF: 4 pages
Proc. SPIE 3740, Optical Engineering for Sensing and Nanotechnology (ICOSN '99), (7 May 1999); doi: 10.1117/12.347844
Show Author Affiliations
Katsumasa Fujita, Osaka Univ. (Japan)
Tomoyuki Kaneko, Osaka Univ. (Japan)
Osamu Nakamura, Osaka Univ. (Japan)
Masahito Oyamada, Kyoto Prefectural Univ. of Medicine (Japan)
Tetsuro Takamatsu, Kyoto Prefectural Univ. of Medicine (Japan)
Satoshi Kawata, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 3740:
Optical Engineering for Sensing and Nanotechnology (ICOSN '99)
Ichirou Yamaguchi, Editor(s)

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