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Proceedings Paper

Laser plasma x-ray contact microscopy of living specimens
Author(s): Alkiviadis Constantinos Cefalas; Panagiotis Argitis; Z. Kollia; Evangelia Sarantopoulou; Thomas W. Ford; Anthony D. Stead; A. Marranca; Colin N. Danson; J. Knott; David Neely
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Paper Abstract

We report on the use of an epoxy novolac chemically amplified photoresist to produce X-ray images of living biological specimens in the water window using laser plasma generated soft X-rays (2.4 - 4.4 nm). The photoresist response was at least one order of magnitude `faster' than the standard PMMA (polymethyl methacrylate) previously used in soft X-ray contact microscopy. After chemical development of the exposed resists, atomic force microscopy (AFM) of the relief images obtained of biological specimens clearly showed the flagella of the motile green alga, Chlamydomonas, suggesting a lateral resolution better than 200 nm, whilst the AFM was capable of discriminating height features of 20 nm in depth profiles.

Paper Details

Date Published: 7 May 1999
PDF: 4 pages
Proc. SPIE 3571, Tenth International School on Quantum Electronics: Laser Physics and Applications, (7 May 1999); doi: 10.1117/12.347658
Show Author Affiliations
Alkiviadis Constantinos Cefalas, National Hellenic Research Foundation (Greece)
Panagiotis Argitis, National Hellenic Research Foundation (Greece)
Z. Kollia, National Hellenic Research Foundation (Greece)
Evangelia Sarantopoulou, National Hellenic Research Foundation (Greece)
Thomas W. Ford, Univ. of London (United Kingdom)
Anthony D. Stead, Univ. of London (United Kingdom)
A. Marranca, Univ. of London (United Kingdom)
Colin N. Danson, Rutherford Appleton Lab. (United Kingdom)
J. Knott, Rutherford Appleton Lab. (United Kingdom)
David Neely, Rutherford Appleton Lab. (United Kingdom)

Published in SPIE Proceedings Vol. 3571:
Tenth International School on Quantum Electronics: Laser Physics and Applications
Peter A. Atanasov; Dimitar V. Stoyanov, Editor(s)

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