Share Email Print
cover

Proceedings Paper

Three-dimensional superresolution imaging of single molecules by spectrally selective confocal microscopy
Author(s): G. J. Brakenhoff; A. M. Van Oijen; J. Kohler; Michiel Muller; J. Schmidt
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

It is shown that based on spectrally selective excitation of individual molecules in the focus of a high NA lens together with position sensitive imaging sub-resolution imaging of three-dimensional structures can be realized. The feasibility of the idea is demonstrated with NA equals 0.55 optics on a model system of pentacene molecules in p-terphenal host matrix.

Paper Details

Date Published: 6 May 1999
PDF: 8 pages
Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); doi: 10.1117/12.347588
Show Author Affiliations
G. J. Brakenhoff, Univ. of Amsterdam (Netherlands)
A. M. Van Oijen, Univ. of Leiden (Netherlands)
J. Kohler, Univ. of Leiden (Netherlands)
Michiel Muller, Univ. of Amsterdam (Netherlands)
J. Schmidt, Univ. of Leiden (Netherlands)


Published in SPIE Proceedings Vol. 3605:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI
Dario Cabib; Carol J. Cogswell; Jose-Angel Conchello; Jeremy M. Lerner; Tony Wilson, Editor(s)

© SPIE. Terms of Use
Back to Top