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Proceedings Paper

Hyperspectral imaging of pathology samples
Author(s): Hiromichi Tsurui; Jeremy M. Lerner; Kuniaki Takahashi; Sachiko Hirose; K. Mitsui; Ko Okumura; Toshikazu Shirai
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Paper Abstract

The object of the experiment described in this paper was to demonstrate that cells stained with multiple fluorophores could be identified and quantified simultaneously. Hyperspectral imaging was used to classify spleen cells of a Balb/c mouse, with the anti-mouse CD4 antibody conjugated with Alexa 488, 532, 546 and 568. It was found that the system was able to identify the specific fluorophore present and map their location in the cells. The system also provided relative signal strength data. Spectral libraries were constructed with color-coded spectra that enabled automatic spectral identification in subsequent acquisitions.

Paper Details

Date Published: 6 May 1999
PDF: 9 pages
Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); doi: 10.1117/12.347573
Show Author Affiliations
Hiromichi Tsurui, Juntendo Univ. School of Medicine (Japan)
Jeremy M. Lerner, LightForm, Inc. (United States)
Kuniaki Takahashi, Holonix International (United States)
Sachiko Hirose, Juntendo Univ. School of Medicine (Japan)
K. Mitsui, Toin Univ. Yokohama (Japan)
Ko Okumura, Juntendo Univ. School of Medicine and CREST Japan (Japan)
Toshikazu Shirai, Juntendo Univ. School of Medicine (Japan)


Published in SPIE Proceedings Vol. 3605:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI
Dario Cabib; Carol J. Cogswell; Jose-Angel Conchello; Jeremy M. Lerner; Tony Wilson, Editor(s)

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