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Proceedings Paper

Effects of scattering in layered biological tissue on imaging spectroscopy data
Author(s): Mark E. Arnoldussen; David Cohen; Gregory H. Bearman; Warren S. Grundfest
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Paper Abstract

Spectral imaging permits two-dimensional mapping of the reflectance properties of biological systems. However, imaging in turbid media involves pixel sizes that are comparable to or smaller than the mean photon path length. This implies that the spectrum measured at a given pixel in the image plane will be determined by manifold photon trajectories through an extended volume in the object, so there is not a uniquely defined path length. In addition, this implies nonlinear spectral mixing for systems with multiple layers and chromophores. Using Monte Carlo model, we have studied photon path distributions in the case of layered turbid systems and their effects on spectral imaging. In particular, we emphasize the effect of hemoglobin on imaging reflectance-mode hyperspectral data.

Paper Details

Date Published: 6 May 1999
PDF: 9 pages
Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); doi: 10.1117/12.347570
Show Author Affiliations
Mark E. Arnoldussen, Univ. of Southern California and Laser Research Lab. (United States)
David Cohen, Laser Research Lab. (United States)
Gregory H. Bearman, Laser Research Lab. and Jet Propulsion Lab. (United States)
Warren S. Grundfest, Laser Research Lab. (United States)

Published in SPIE Proceedings Vol. 3605:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI
Dario Cabib; Carol J. Cogswell; Jose-Angel Conchello; Jeremy M. Lerner; Tony Wilson, Editor(s)

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