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Proceedings Paper

Optical sectioning using finite-sized multiple-aperture arrays in fluorescence direct-view microscopy: experimental results
Author(s): Patrick J. Smith; Cian M. Taylor; Eithne M. McCabe
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Paper Abstract

Confocal fluorescence imaging is widely used, particularly for biological applications, and also notably in direct-view microscopes. Recent work has compared the use of coherent and incoherent illumination sources on the optical sectioning characteristics of fluorescence direct-view microscopes. However this detailed comparison has been done in theory. This paper addresses the experimental aspects of using coherent light sources in fluorescence imaging using a range of finite- sized, multiple-aperture arrays. The experimental difficulties of choosing a suitable uniform, flat, fluorescent plane with a high quantum efficiency are considered. Axial response curves obtained with a fluorescent laser dye sample are presented.

Paper Details

Date Published: 6 May 1999
PDF: 5 pages
Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); doi: 10.1117/12.347569
Show Author Affiliations
Patrick J. Smith, Trinity College Dublin (Ireland)
Cian M. Taylor, Trinity College Dublin (Ireland)
Eithne M. McCabe, Trinity College Dublin (Ireland)


Published in SPIE Proceedings Vol. 3605:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI
Dario Cabib; Carol J. Cogswell; Jose-Angel Conchello; Jeremy M. Lerner; Tony Wilson, Editor(s)

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