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Proceedings Paper

Spatial and temporal single-cell volume estimation by a fluorescence imaging technique with application to astrocytes in primary culture
Author(s): Siamak Khatibi; Louise Allansson; Tomas Gustavsson; Fredrik Blomstrand; Elisabeth Hansson; Torsten Olsson
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Paper Abstract

Cell volume changes are often associated with important physiological and pathological processes in the cell. These changes may be the means by which the cell interacts with its surrounding. Astroglial cells change their volume and shape under several circumstances that affect the central nervous system. Following an incidence of brain damage, such as a stroke or a traumatic brain injury, one of the first events seen is swelling of the astroglial cells. In order to study this and other similar phenomena, it is desirable to develop technical instrumentation and analysis methods capable of detecting and characterizing dynamic cell shape changes in a quantitative and robust way. We have developed a technique to monitor and to quantify the spatial and temporal volume changes in a single cell in primary culture. The technique is based on two- and three-dimensional fluorescence imaging. The temporal information is obtained from a sequence of microscope images, which are analyzed in real time. The spatial data is collected in a sequence of images from the microscope, which is automatically focused up and down through the specimen. The analysis of spatial data is performed off-line and consists of photobleaching compensation, focus restoration, filtering, segmentation and spatial volume estimation.

Paper Details

Date Published: 6 May 1999
PDF: 10 pages
Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); doi: 10.1117/12.347564
Show Author Affiliations
Siamak Khatibi, Chalmers Univ. of Technology (Sweden)
Louise Allansson, Goeteborg Univ. (Sweden)
Tomas Gustavsson, Chalmers Univ. of Technology (Sweden)
Fredrik Blomstrand, Goeteborg Univ. (Sweden)
Elisabeth Hansson, Goeteborg Univ. (Sweden)
Torsten Olsson, Chalmers Univ. of Technology (Sweden)


Published in SPIE Proceedings Vol. 3605:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI
Dario Cabib; Carol J. Cogswell; Jose-Angel Conchello; Jeremy M. Lerner; Tony Wilson, Editor(s)

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