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Proceedings Paper

Spatial point spread function with depth in two-photon microscopy
Author(s): Andrew K. Dunn; Vincent P. Wallace; Mariah L. Coleno; Peter T. C. So; Bruce J. Tromberg
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Paper Abstract

The spatial point spread function is studied for two-photon fluorescence imaging in turbid media. Using a Monte Carlo based model the effects of tissue optical properties on the point spread function, fluorescence generation, and fluorescence collection are studied. The simulated results are compared to measured point spread functions at different depths within a scattering sample. Results indicate that the limiting factor in two-photon imaging is a loss of signal caused by scattering rather than a loss of resolution.

Paper Details

Date Published: 6 May 1999
PDF: 8 pages
Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); doi: 10.1117/12.347559
Show Author Affiliations
Andrew K. Dunn, Univ. of California/Irvine (United States)
Vincent P. Wallace, Univ. of California/Irvine (United Kingdom)
Mariah L. Coleno, Univ. of California/Irvine (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)
Bruce J. Tromberg, Univ. of California/Irvine (United States)

Published in SPIE Proceedings Vol. 3605:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI
Dario Cabib; Carol J. Cogswell; Jose-Angel Conchello; Jeremy M. Lerner; Tony Wilson, Editor(s)

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