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Proceedings Paper

Use of the optical quadrature method in tomographic microscopy
Author(s): Yan Glina; George A. Tsihrintzis; Carol M. Warner; Daniel O. Hogenboom; Charles A. DiMarzio
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Paper Abstract

The optical quadrature imaging technique, as derived and extended from microwave and laser radar quadrature detection techniques, provides an efficient method for obtaining phase information from a sample that has little or no amplitude contrast. We are able to resolve internal structures of a sample that are defined by relatively small refractive index differences without the use of dyes or stains, while using much lower light levels than conventional techniques. We have constructed a prototype system for imaging microscopic phase- only objects. In this paper, we present its capabilities, as well as the imaging and reconstruction methods used to obtain quantitative information about a sample.

Paper Details

Date Published: 6 May 1999
PDF: 6 pages
Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); doi: 10.1117/12.347557
Show Author Affiliations
Yan Glina, Northeastern Univ. (United States)
George A. Tsihrintzis, Northeastern Univ. (United States)
Carol M. Warner, Northeastern Univ. (United States)
Daniel O. Hogenboom, Northeastern Univ. (United States)
Charles A. DiMarzio, Northeastern Univ. (United States)

Published in SPIE Proceedings Vol. 3605:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI
Dario Cabib; Carol J. Cogswell; Jose-Angel Conchello; Jeremy M. Lerner; Tony Wilson, Editor(s)

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