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Proceedings Paper

Fluorescence decay kinetics and localization of disulphonated aluminium phthalocyanine in fibroblasts: a confocal fluorescence microscopy study
Author(s): Zdenek Petrasek; Richard B. Ostler; Ilya V. Eigenbrot; David Phillips
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Paper Abstract

Steady state and time resolved confocal fluorescence microscopy, using a point scanning system, is applied to an investigation of the early stages of photo-induced changes in 3T3-L1 murine fibroblasts using di-sulphonated aluminum phthalocyanine (AlPcS2) as a photosensitizer. A comparison is made with data obtained using a line scan system and V79-4 Chinese hamster fibroblasts. The steady state data obtained in this work demonstrate that intracellular AlPcS2 fluorescence intensity increases progressively on photoirradiation. Time-resolved studies indicate that this could result from a progressive decrease in the concentration of the self-quenched membrane-associated form of AlPcS2 following its conversion into the fluorescent monomeric form.

Paper Details

Date Published: 3 May 1999
PDF: 12 pages
Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); doi: 10.1117/12.347547
Show Author Affiliations
Zdenek Petrasek, Imperial College of Science, Medicine and Technology (United Kingdom)
Richard B. Ostler, Imperial College of Science, Medicine and Technology (United Kingdom)
Ilya V. Eigenbrot, Imperial College of Science, Medicine and Technology (United Kingdom)
David Phillips, Imperial College of Science, Medicine and Technology (United Kingdom)


Published in SPIE Proceedings Vol. 3602:
Advances in Fluorescence Sensing Technology IV
Joseph R. Lakowicz; Steven A. Soper; Richard B. Thompson, Editor(s)

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