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Proceedings Paper

Array biosensor: recent developments
Author(s): Joel P. Golden; Chris A. Rowe-Taitt; Mark J. Feldstein; Frances S. Ligler
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Paper Abstract

A fluorescence-based immunosensor has been developed for simultaneous analyses of multiple samples for 1 to 6 different antigens. A patterned array of recognition antibodies immobilized on the surface of a planar waveguide is used to 'capture' analyte present in samples. Bound analyte is then quantified by means of fluorescent detector molecules. Upon excitation of the fluorescent label by a small diode laser, a CCD camera detects the pattern of fluorescent antigen:antibody complexes on the sensor surface. Image analysis software correlates the position of fluorescent signals with the identity of the analyte. A new design for a fluidics distribution system is shown, as well as results from assays for physiologically relevant concentrations of staphylococcal enterotoxin B (SEB), F1 antigen from Yersinia pestis, and D- dimer, a marker of sepsis and thrombotic disorders.

Paper Details

Date Published: 3 May 1999
PDF: 7 pages
Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); doi: 10.1117/12.347546
Show Author Affiliations
Joel P. Golden, Naval Research Lab. (United States)
Chris A. Rowe-Taitt, Naval Research Lab. (United States)
Mark J. Feldstein, Naval Research Lab. (United States)
Frances S. Ligler, Naval Research Lab. (United States)


Published in SPIE Proceedings Vol. 3602:
Advances in Fluorescence Sensing Technology IV
Joseph R. Lakowicz; Steven A. Soper; Richard B. Thompson, Editor(s)

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