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Proceedings Paper

Spectral properties and biological applications of solvatochromic Dapoxyl dyes
Author(s): Zhenjun Diwu; Yixin Lu; Cailan Zhang; Dieter H. Klaubert; Richard P. Haugland
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Paper Abstract

Fluorescent molecules, whose spectra or quantum yields are sensitive to their surrounding environments, are valuable in the study of heterogeneous media, organized media and biological media, and many fluorescent solvatochromic dyes have been developed for these applications. However, the existing fluorescent solvatochromic dyes either have short absorption and emission wavelengths, low extinction coefficients, low quantum yields or small Stokes shift. We have recently developed solvatochromic DapoxylTM dyes, whose fluorescence maximum shifts to longer wavelengths, and fluorescence quantum yield decreases with increasing solvent polarity. In these molecules, there is a 'push-pull' electron transfer system from the 5-phenyl moiety to the 2-phenyl ring. The compounds show strong solvent-dependent fluorescence that is well correlated with the empirical solvent polarity parameter ET(30). These dyes have also shown long emission wavelengths, high extinction coefficients, high quantum yields and large Stokes shifts. Their biological applications will also be discussed.

Paper Details

Date Published: 3 May 1999
PDF: 9 pages
Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); doi: 10.1117/12.347529
Show Author Affiliations
Zhenjun Diwu, Molecular Probes, Inc. (United States)
Yixin Lu, Molecular Probes, Inc. (United States)
Cailan Zhang, Molecular Probes, Inc. (United States)
Dieter H. Klaubert, Molecular Probes, Inc. (United States)
Richard P. Haugland, Molecular Probes, Inc. (United States)


Published in SPIE Proceedings Vol. 3602:
Advances in Fluorescence Sensing Technology IV
Joseph R. Lakowicz; Steven A. Soper; Richard B. Thompson, Editor(s)

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