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Proceedings Paper

Monitoring tissue metabolism via time-resolved laser fluorescence
Author(s): Holger K. Maerz; Rainer Buchholz; Frank Emmrich; Frank Fink; Clive L. Geddes; Lutz Pfeifer; Ferdinand Raabe; Uwe Marx
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Paper Abstract

Most assays for drug screening are monitoring the metabolism of cells by detecting the NADH content, which symbolize its metabolic activity, indirectly. Nowadays, the performance of a LASER enables us to monitor the metabolic state of mammalian cells directly and on-line by using time-resolved autofluorescence detection. Therefore, we developed in combination with tissue engineering, an assay for monitoring minor toxic effects of volatile organic compounds (VOC), which are accused of inducing Sick Building Syndrome (SBS). Furthermore, we used the Laserfluoroscope (LF) for pharmacological studies on human bone marrow in vitro with special interest in chemotherapy simulation. In cancer research and therapy, the effect of chemostatica in vitro in the so-called oncobiogram is being tested; up to now without great success. However, it showed among other things that tissue structure plays a vital role. Consequently, we succeeded in simulating a chemotherapy in vitro on human bone marrow. Furthermore, after tumor ektomy we were able to distinguish between tumoric and its surrounding healthy tissue by using the LF. With its sensitive detection of metabolic changes in tissues the LF enables a wide range of applications in biotechnology, e.g. for quality control in artificial organ engineering or biocompatability testing.

Paper Details

Date Published: 3 May 1999
PDF: 12 pages
Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); doi: 10.1117/12.347527
Show Author Affiliations
Holger K. Maerz, Heart Ctr. Leipzig GmbH/Univ. Leipzig (Germany)
Rainer Buchholz, Technical Univ. Berlin (Germany)
Frank Emmrich, Univ. Leipzig (Germany)
Frank Fink, College for Technology and Economy Berlin (Germany)
Clive L. Geddes, Innovative Optische Messtechnik GmbH (Germany)
Lutz Pfeifer, Innovative Optische Messtechnik GmbH (Germany)
Ferdinand Raabe, Univ. Leipzig (Germany)
Uwe Marx, Univ. Leipzig (Germany)

Published in SPIE Proceedings Vol. 3602:
Advances in Fluorescence Sensing Technology IV
Joseph R. Lakowicz; Steven A. Soper; Richard B. Thompson, Editor(s)

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