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Proceedings Paper

Evaluation of a red fluorescent dye for high-throughput compound screening applications in pharmaceutical discovery research
Author(s): Kerry M. Swift; Edmund D. Matayoshi
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Paper Abstract

The possibility and potential benefits of using an extreme red fluorescent dye such as Cy5.5TM to label drug discovery target proteins was studied experimentally. Cy5.5 labeled BSA, GFP, and CRP were used as examples of protein-dye conjugates whose binding to corresponding antibody was detected by changes in either rotational or transnational diffusion properties, that is, by either fluorescence polarization or confocal fluorescence correlation spectroscopy (FCS). In addition, FCS was used to quantitate excitation of Cy5.5 to its triplet state. Fluorescence polarization and lifetime were measured as a function of excitation wavelength or glycerol concentration and solvent viscosity.

Paper Details

Date Published: 3 May 1999
PDF: 7 pages
Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); doi: 10.1117/12.347522
Show Author Affiliations
Kerry M. Swift, Abbott Labs. (United States)
Edmund D. Matayoshi, Abbott Labs. (United States)


Published in SPIE Proceedings Vol. 3602:
Advances in Fluorescence Sensing Technology IV
Joseph R. Lakowicz; Steven A. Soper; Richard B. Thompson, Editor(s)

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