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Proceedings Paper

Fluorescence correlation spectroscopy with traveling interference fringe excitation apparatus using AOM devices for biological applications
Author(s): Mineyuki Hattori; Hideaki Shimizu
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Paper Abstract

A new improved fluorescence correlation spectroscopy apparatus for determining motional states of particles, in which the scheme of fluorescence correlation spectroscopy with traveling interference fringe excitation (FCSTFE) is applied, is presented. In this method, the modulated fluorescence signal from particles excited by a moving interference fringe is detected, and cosine and sine Fourier coefficients at the frequency of the traveling fringe are recorded. The autocorrelation function of the fluorescence intensity consists of terms which are characterized by the size of the excitation region and the spacing of the fringe. A lock-in detection method is adopted for extracting cosine and sine Fourier coefficients at the frequency of the traveling fringe [Fcos(t), Fsin(t)]. Autocorrelation functions of Fcos(t) and Fsin(t) express the motion of the fluorescent particles. We constructed the apparatus using acousto-optic modulator (AOM) devices to generate the traveling fringes. The potential of this method for the extraction of information about the dynamics of particles in biological systems is discussed.

Paper Details

Date Published: 3 May 1999
PDF: 9 pages
Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); doi: 10.1117/12.347512
Show Author Affiliations
Mineyuki Hattori, Electrotechnical Lab. (Japan)
Hideaki Shimizu, Electrotechnical Lab. (Japan)


Published in SPIE Proceedings Vol. 3602:
Advances in Fluorescence Sensing Technology IV
Joseph R. Lakowicz; Steven A. Soper; Richard B. Thompson, Editor(s)

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