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Proceedings Paper

Comparison between a conventional epifluorescence microscope and a new highly efficient evanescent wave detector in single-molecule spectroscopic applications
Author(s): Joerg Enderlein; Thomas Ruckstuhl; Frank Loescher; Martin Boehmer; Stefan Seeger
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Paper Abstract

We present a comparison between two basically different optical detection systems: a confocal epifluorescence microscope, and a new evanescent wave detection system employing a parabolic optical element. In a microscope set-up, fluorescence light is collected within a cone around the optical axis, whereas in the evanescent light detector, fluorescence light is collected mainly at angles larger than the so-called critical angle of total internal reflection. Based on a thorough theoretical modeling of both experimental set-ups, comparison between the two detection systems is made Particularly, the optical detection efficiency is compared. 5

Paper Details

Date Published: 3 May 1999
PDF: 8 pages
Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); doi: 10.1117/12.347511
Show Author Affiliations
Joerg Enderlein, Univ. Regensburg (Germany)
Thomas Ruckstuhl, Univ. Regensburg (Germany)
Frank Loescher, Univ. Regensburg (Germany)
Martin Boehmer, Univ. Regensburg (Germany)
Stefan Seeger, Univ. Regensburg (Switzerland)

Published in SPIE Proceedings Vol. 3602:
Advances in Fluorescence Sensing Technology IV
Joseph R. Lakowicz; Steven A. Soper; Richard B. Thompson, Editor(s)

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