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Proceedings Paper

Subcellular optical coherence tomography with a Kerr lens mode-locked Ti:Al2O3 laser
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Paper Abstract

The longitudinal resolution of optical coherence tomography (OCT) is currently limited by the optical bandwidth of the light source, typically a superluminescent diodes, to approximately 10-15 micrometers . This resolution is insufficient to identify individual cells or to assess subcellular structures such as nuclei or mitotic figures. The ability to perform subcellular imaging with OCT could greatly enhance the detection of early neoplastic changes and improve early cancer diagnosis or the imaging of developing biological morphology. Higher resolution OCT would also improve specificity of diagnosis for several ocular diseases, such as glaucoma, which require precise, detailed imaging and measurement of retinal nerve fiber layer thickness. State of the art Kerr-lens mode-locked Ti:Al2O3 lasers using double chirped dispersion compensating mirrors can generate pulse durations of < 7 fs and bandwidths of 200 nm or more at 800 nm center wavelength. These pulse durations and bandwidths can be used for OCT, resulting in longitudinal resolutions of less than 2 micrometers . The use of such broad bandwidths also enables the extraction of localized, wavelength dependent absorption and scattering tissue characteristic by detecting the full interferometric fringe signa and using Fourier signal processing. In this paper we demonstrate an ultra-high-subcellular level resolution, spectroscopic OCT system based on a mode-locked Ti:Al2O3 laser. In vivo imaging of development biology specimens as well as preliminary in vivo spectroscopic OCT result are demonstrated.

Paper Details

Date Published: 30 April 1999
PDF: 8 pages
Proc. SPIE 3598, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications III, (30 April 1999); doi: 10.1117/12.347502
Show Author Affiliations
Wolfgang Drexler, Massachusetts Institute of Technology (Austria)
Uwe Morgner, Massachusetts Institute of Technology (Germany)
Constantinos Pitris, Massachusetts Institute of Technology (United States)
Stephen A. Boppart, Massachusetts Institute of Technology (United States)
Franz X. Kaertner, Massachusetts Institute of Technology (United States)
Xing De Li, Massachusetts Institute of Technology (United States)
Seong-Ho Cho, Massachusetts Institute of Technology (United States)
Erich P. Ippen, Massachusetts Institute of Technology (United States)
Mark E. Brezinski, Massachusetts General Hospital and Harvard Medical School (United States)
James G. Fujimoto, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 3598:
Coherence Domain Optical Methods in Biomedical Science and Clinical Applications III
Valery V. Tuchin; Joseph A. Izatt, Editor(s)

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