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Proceedings Paper

Laser-scan cytometry: a new tool for clinical diagnostics
Author(s): Holger K. Maerz; Adolf Baumgartner; Joerg Hambsch; Bert Hennig; Michael Nuesse; Thomas Schmid; Peter Schneider; Rainer Zotz; Attila Tarnok
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Paper Abstract

The common usage of flow cytometry (FCM) in research and clinical diagnostic is limited by the lack visualizing the fluorescence labelled cells. The Laser Scanning Cytometer (LSC) enables multicolor cytometric measurements on a slide featuring relocation of single cells for further investigation via brightfield and fluorescence microscopy. Additionally, it is possible to capture these images for documentation. In a FISH application, the LSC was successfully used for automated scoring techniqeus for evaluating the frequency of aneuploid sperm in humans and mice. In just 30 minutes, we were able to acquire more than 15,000 sperms, a task which normally takes more than a day. After relocation, genetic defects were identified and confirmed via fluorescence microscopy. In an on going study, we investigate via the LSC the remain of a new radiopaque material for high resolution echocardiography in the blood circulation. At first the result exhibited that the radiopaque material is endocysed by leukocytes just after application but is still detectable via echocardiography for up to 40 minutes. In conclusion, with the additional data acquisition by the LSC, it is possible to perform further detailed information from very small samples. Therefore, we are working up to now on developing new methods to introduce the LSC in our clinical diagnostic of neonates undergoing cardiac surgery.

Paper Details

Date Published: 21 April 1999
PDF: 11 pages
Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); doi: 10.1117/12.346761
Show Author Affiliations
Holger K. Maerz, Univ. Hospital Leipzig (Germany)
Adolf Baumgartner, GSF Research Ctr. (Germany)
Joerg Hambsch, Univ. Hospital Leipzig (Germany)
Bert Hennig, Univ. Hospital Leipzig (Germany)
Michael Nuesse, GSF Research Ctr. (Germany)
Thomas Schmid, GSF Research Ctr. (Germany)
Peter Schneider, Univ. Hospital Leipzig (Germany)
Rainer Zotz, Univ. Hospital Leipzig (Germany)
Attila Tarnok, Univ. Hospital Leipzig (Germany)

Published in SPIE Proceedings Vol. 3603:
Systems and Technologies for Clinical Diagnostics and Drug Discovery II
Gerald E. Cohn; John C. Owicki, Editor(s)

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