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Proceedings Paper

Use of a Fluorometric Imaging Plate Reader in high-throughput screening
Author(s): Duncan R. Groebe; Sujatha Gopalakrishnan; Holly Hahn; Usha Warrior; Linda Traphagen; David J. Burns
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Paper Abstract

High-throughput screening (HTS) efforts at Abbott Laboratories have been greatly facilitated by the use of a Fluorometric Imaging Plate Reader. The FLIPR consists of an incubated cabinet with integrated 96-channel pipettor and fluorometer. An argon laser is used to excite fluorophores in a 96-well microtiter plate and the emitted fluorometer. An argon laser is used to excite fluorophores in a 96-well microtiter plate and the emitted fluorescence is imaged by a cooled CCD camera. The image data is downloaded from the camera and processed to average the signal form each well of the microtiter pate for each time point. The data is presented in real time on the computer screen, facilitating interpretation and trouble-shooting. In addition to fluorescence, the camera can also detect luminescence form firefly luciferase.

Paper Details

Date Published: 21 April 1999
PDF: 10 pages
Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); doi: 10.1117/12.346753
Show Author Affiliations
Duncan R. Groebe, Abbott Labs. (United States)
Sujatha Gopalakrishnan, Abbott Labs. (United States)
Holly Hahn, Abbott Labs. (United States)
Usha Warrior, Abbott Labs. (United States)
Linda Traphagen, Abbott Labs. (United States)
David J. Burns, Abbott Labs. (United States)


Published in SPIE Proceedings Vol. 3603:
Systems and Technologies for Clinical Diagnostics and Drug Discovery II
Gerald E. Cohn; John C. Owicki, Editor(s)

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