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Proceedings Paper

Frequency-domain pump-probe microscopic imaging using intensity-modulated laser diodes
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Paper Abstract

We report the implementation of intensity modulated diode lasers in frequency-domain pump-probe studies, diode lasers are compact, stable, and economical units that require little maintenance. In our study, a 365 nm diode laser is used as the excitation source and the output of a 680 nm unit induces stimulated emission from excited state fluorophores. By modulating the intensities of the two diode lasers at slightly different frequencies, and detecting the fluorescence signal at the cross-correlation frequency, both time-resolved and high spatial resolution imaging can be achieved. The laser diodes are modulated in the 100 MHz cross-correlation signal has been used for time-resolved imaging of fluorescent microspheres and mouse fibroblasts labeled with nucleic acid stains TOTO-3. These results demonstrate and feasibility of using intensity modulated diode lasers for frequency-domain, pump-probe studies.

Paper Details

Date Published: 21 April 1999
PDF: 10 pages
Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); doi: 10.1117/12.346749
Show Author Affiliations
Christof Buehler, Massachusetts Institute of Technology (Switzerland)
Chen-Yuan Dong, Massachusetts Institute of Technology (Taiwan)
Peter T. C. So, Massachusetts Institute of Technology (United States)
Todd E. French, LJL BioSystems, Inc. (United States)
Enrico Gratton, Univ. of Illinois/Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 3603:
Systems and Technologies for Clinical Diagnostics and Drug Discovery II
Gerald E. Cohn; John C. Owicki, Editor(s)

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