Share Email Print
cover

Proceedings Paper

Improved fluorophores for zinc biosensing using carbonic anhydrase
Author(s): Richard B. Thompson; Badri P. Maliwal; Hui-Hui Zeng
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Previously, we had shown that the zinc-dependent binding of certain fluorescent aryl sulfonamide inhibitors could be used with apo-carbonic anhydrase II to transduce the level of free zinc as a change in the fluorescence of the inhibitor. While inhibitors such as dansylamide, ABD-M, and ABD-N made possible quantitation of free zinc in the picomolar range with high selectivity, they have only modest absorbance which limits their utility. We describe here the synthesis and properties of two new probes, Dapoxyl sulfonamide and BTCS, and their use in zinc biosensing. Dapoxyl sulfonamide exhibits a dramatic increase and blue shift in its emission upon binding to holo-carbonic anhydrase II, as well as a twenty-fold increase in lifetime: it is thus well suited for quantitating free Zn(II) down to picomolar ranges. The anisotropy of BTCS increases five-fold binding to the holoprotein making this probe well suited for anisotropy-based determination of zinc.

Paper Details

Date Published: 21 April 1999
PDF: 9 pages
Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); doi: 10.1117/12.346738
Show Author Affiliations
Richard B. Thompson, Univ. of Maryland School of Medicine/Baltimore (United States)
Badri P. Maliwal, Univ. of Maryland School of Medicine/Baltimore (United States)
Hui-Hui Zeng, Univ. of Maryland School of Medicine/Baltimore (United States)


Published in SPIE Proceedings Vol. 3603:
Systems and Technologies for Clinical Diagnostics and Drug Discovery II
Gerald E. Cohn; John C. Owicki, Editor(s)

© SPIE. Terms of Use
Back to Top