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Proceedings Paper

Tumor diagnostics using fiber optical IR spectroscopy
Author(s): Harald Winter; Uwe Bindig; Wolfgang Waesche; K. Liebold; Andre Roggan; P. Frege; Ulrich M. Gross; G. Mueller
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Paper Abstract

Aim of the project is the development of an in vivo endoscopic method to differentiate between cancerous from healthy tissue. The method is based on IR spectra in which each diseased state of the tissue has its own characteristic pattern as already shown in previous experiments. Two regions (1245 - 1195) cm-1 and (1045 - 995) cm-1 within the fingerprint (less than 1500 cm-1) region were selected for analysis. This paper will present the technical design of the laboratory set up and outcome of the development as well as the experiments. Two lead-salt diode lasers were used as excitation sources. The IR-radiation was transmitted via silverhalide fibers to the tissue to be investigated. On the detection side another IR fiber was used to transmit the signal to an MCT-detector (Mercury-Cadmium-Telluride). Measurement modes are Attenuated Total Reflectance (ATR) and diffuse Reflection/Remission. Spatial resolution was 100 X 100 micrometer2. The tissue used for these experiments was human colon carcinoma under humidity conditions. Samples were mapped using a stepper motor powered x/y/z-translation stage with a resolution of 1 micrometer. Results were compared with measurements carried out using a FTIR-interferometer and an FTIR-microscope in the region from 4000 - 900 cm-1. Soft- and Hardware control of the experiment is done using Labwindows/CVI (National Instruments, USA).

Paper Details

Date Published: 21 April 1999
PDF: 13 pages
Proc. SPIE 3596, Specialty Fiber Optics for Medical Applications, (21 April 1999); doi: 10.1117/12.346716
Show Author Affiliations
Harald Winter, Laser- und Medizin-Technologie gGmbH (Germany)
Uwe Bindig, Laser- und Medizin-Technologie gGmbH (Germany)
Wolfgang Waesche, Universitaetsklinikum Charite der Humboldt-Univ. zu Berlin (Germany)
K. Liebold, Laser- und Medizin-Technologie gGmbH (Germany)
Andre Roggan, Universitaetsklinikum Benjamin Franklin der Freie Univ. Berlin (Germany)
P. Frege, Universitaetsklinikum Benjamin Franklin der Freie Univ. Berlin (Germany)
Ulrich M. Gross, Universitaetsklinikum Benjamin Franklin der Freie Univ. Berlin (Germany)
G. Mueller, Laser- und Medizin-Technologie gGmbH and Universitaetsklinikum Benjamin Franklin der Freie (Germany)

Published in SPIE Proceedings Vol. 3596:
Specialty Fiber Optics for Medical Applications
Abraham Katzir; James A. Harrington, Editor(s)

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