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Proceedings Paper

Human coronary atherosclerosis studied in vitro by catheter-based transluminal Raman spectroscopy
Author(s): Hendrik P. J. Buschman; Tjeerd J. Romer; Michael L. Wach; Eric Marple; Arnoud van der Laarse; Albert V.G. Bruschke; Gerwin J. Puppels
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Paper Abstract

We used Raman spectroscopy to assess the chemical composition and pathological states of atherosclerosis in in vitro intact human coronary artery tissue. Human coronary artery samples expressing different stages of atherosclerosis were mounted in an in vitro set-up and perfused with a salt solution. NIR laser light was delivered to the tissue through the central fiber of an optical fiber catheter that was inserted transluminally into a buffer- perfused arterial segment. Tissue Raman signal was collected by seven fibers surrounding the central fiber. The collected Raman light was launched into a spectrometer and imaged onto a CCD. High signal to noise, low background tissue Raman spectra were obtained in 10-60 s form artery samples. The spectral information from each collection fiber was linearly modeled with a Raman spectral model that quantifies the chemical composition of the arterial wall. The model results showed excellent fits to all Raman spectra. A diagnostic algorithm, that has proven to have excellent correlation with historilogical classification by a pathologist, classified the examined tissue into one of three pathological states. From these experiments we conclude that intravascular optical fiber Raman spectroscopy can provide in situ histopathology, which may be used to study vascular disease in vivo.

Paper Details

Date Published: 9 April 1999
PDF: 6 pages
Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); doi: 10.1117/12.345409
Show Author Affiliations
Hendrik P. J. Buschman, Leiden Univ. Medical Ctr. and Erasmus Univ. Rotterdam and Univ. Hospital Rotterdam (Netherlands)
Tjeerd J. Romer, Leiden Univ. Medical Ctr. and Erasmus Univ. Rotterdam and Univ. Hospital Rotterdam (Netherlands)
Michael L. Wach, Visionex, Inc. (United States)
Eric Marple, Visionex, Inc. (United States)
Arnoud van der Laarse, Leiden Univ. Medical Ctr. (Netherlands)
Albert V.G. Bruschke, Leiden Univ. Medical Ctr. (Netherlands)
Gerwin J. Puppels, Erasmus Univ. Rotterdam and Univ. Hospital Rotterdam (Netherlands)

Published in SPIE Proceedings Vol. 3608:
Biomedical Applications of Raman Spectroscopy
Michael D. Morris, Editor(s)

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