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Proceedings Paper

Surface-enhanced Raman scattering spectra of TvNPV polyhedrin protein in Ag sol
Author(s): PeiDi Bao; XinMing Liu; TianQuan Huang; Tieqiao Wu; Shiqui Liu
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Paper Abstract

The surface-enhanced Raman Scattering (SERS) spectra of insect virus: the Polyhedrin Protein and Trabala Vishnou Nuclear Polyhedrosis Virus (TvNPV-PP) adsorbed on the surface of silver colloid particles and their Ordinary Raman Scattering (ORS) spectrum were investigated. The SERS spectra and the ORS spectrum of TvNPV-PP show their correlation. In ORS spectrum of TvNPV-PP. The intense amide I at 1656 nm-1, the medium-intense amide I 1673 cm-1 and a week amide III at 1280 cm-1 show that TvNPV-PP has predominantly (alpha) -helical and (beta) - sheet structure. In SERS spectra of TvNPV-PP the intense bands related to carboxylate groups vibrations suggest that TvNPV-PP are adsorbed on the silver colloid surface through the carboxylate groups. The bands of amide I and amide III could not be observed in SERS residues increased their distance from Ag surface and weaken the enhanced effect of the corresponding vibrations. Chemisorption is a main mechanism in the SERS spectra on silver hydrosols for TvNPV- PP and its mechanism of enhancement has a short-range character. The SERS enhancement factor is approximately 102.

Paper Details

Date Published: 9 April 1999
PDF: 6 pages
Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); doi: 10.1117/12.345406
Show Author Affiliations
PeiDi Bao, Sichuan Univ. (China)
XinMing Liu, Sichuan Univ. (China)
TianQuan Huang, Sichuan Univ. (China)
Tieqiao Wu, Sichuan Univ. (China)
Shiqui Liu, Sichuan Univ. (China)


Published in SPIE Proceedings Vol. 3608:
Biomedical Applications of Raman Spectroscopy
Michael D. Morris, Editor(s)

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