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Proceedings Paper

Human coronary atherosclerosis studied by morphological NIR Raman confocal microspectroscopy
Author(s): Hendrik P. J. Buschman; Jason T. Motz; Maryann Fitzmaurice; Arnoud van der Laarse; Albert V.G. Bruschke; Michael S. Feld
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Paper Abstract

We used Raman microspectroscopy to study all major morphological structures from normal and atherosclerotic tissue expressing different stages of disease. Thin sections from coronary artery sample;s were studied with a Raman microspectrometer system. Raman spectra were collected from the internal and external elastic lamina, collagen fibers/fibrous cap, foam cells, smooth muscle cells, necrotic core, adventitial fat, (beta) -carotene containing crystal, cholesterol crystals and calcium mineralizations. To assess the chemical composition of the examined morphological structures each spectrum was modeled with a chemical algorithm containing the Raman spectra of the major chemicals present in artery wall. The results of this analysis show that the chemical composition of each morphological structure is well defined and shows little variation between similar structures from different samples.

Paper Details

Date Published: 9 April 1999
PDF: 5 pages
Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); doi: 10.1117/12.345400
Show Author Affiliations
Hendrik P. J. Buschman, Leiden Univ. Medical Ctr. and Massachusetts Institute of Technology (Netherlands)
Jason T. Motz, Massachusetts Institute of Technology (United States)
Maryann Fitzmaurice, Univ. Hospitals of Cleveland and Case Western Univ. (United States)
Arnoud van der Laarse, Leiden Univ. Medical Ctr. (Netherlands)
Albert V.G. Bruschke, Leiden Univ. Medical Ctr. (Netherlands)
Michael S. Feld, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 3608:
Biomedical Applications of Raman Spectroscopy
Michael D. Morris, Editor(s)

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