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Proceedings Paper

Raman and SERS microspectroscopy on living cells: a promising tool toward cellular drug response and medical diagnosis
Author(s): Abdelilah Beljebbar; Ganesh D. Sockalingum; Hamid Morjani; Michel Manfait
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Paper Abstract

Raman spectroscopy has been sued to differentiate between sensitive and MDR-resistant cells using Raman spectral imaging with a 632.8 nm excitation wavelength. The comparison between two spectral images allowed to quantify the differences between sensitive and resistant cell lines in term of proteins, lipids when MDR phenotype is expressed. SER spectroscopy has become a powerful and non-invasive probe for investigating the molecular and cellular interaction of drugs with their targets. The comparison between these models allow to elucidate the biological effect of the drugs. The development of new types of SERS- active substrates has extended the applicability of this technique to medical diagnosis. Two kinds of SERS active substrates, characterized as 'bio-compatible' systems, can be used for investigation on single living cells: colloid suspensions and microelectrodes and island films. This methodology is used for the study of cell membrane components in interaction with the SERS substrates with the aim to understand the resistance mechanism. The constitution of a data bank will allow the follow-up of cancer and future monitoring of therapeutic intervention.

Paper Details

Date Published: 9 April 1999
PDF: 10 pages
Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); doi: 10.1117/12.345399
Show Author Affiliations
Abdelilah Beljebbar, Univ. de Reims Champagne-Ardenne (Netherlands)
Ganesh D. Sockalingum, Univ. de Reims Champagne-Ardenne (France)
Hamid Morjani, Univ. de Reims Champagne-Ardenne (France)
Michel Manfait, Univ. de Reims Champagne-Ardenne (France)

Published in SPIE Proceedings Vol. 3608:
Biomedical Applications of Raman Spectroscopy
Michael D. Morris, Editor(s)

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