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Proceedings Paper

Noninvasive assessment of ocular tissue using confocal Raman spectroscopy
Author(s): James P. Wicksted; Noel J.C. Bauer; Roel J. Erckens; Franciscus H. M. Jongsma; Eefke E. Smit-Kepel; Massoud Motamedi; Wayne F. March
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Paper Abstract

We have developed a confocal Raman spectroscopy system in order to noninvasively characterize ocular tissue with both an in vitro nd in vivo capability. This systems consists of a long working distance optical probe, which focuses the incident laser light on the tissue and collects the backscattered Raman signal, a single grating spectrometer with CCD camera, and an optical fiber which couples the optical probe to the spectrometer. Essential to the confocal design is that the sample volume is limited, preventing detection of interference signals and fluorescence from adjacent ocular tissues. This sample volume is adjustable by changing the diameter of the collection fiber which acts as the pinhole in the system. Potential applications of this technique such as assessing corneal hydration and quantifying pharmacokinetic drug transport across the cornea will be discussed.

Paper Details

Date Published: 9 April 1999
PDF: 11 pages
Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); doi: 10.1117/12.345392
Show Author Affiliations
James P. Wicksted, Oklahoma State Univ. (United States)
Noel J.C. Bauer, Univ. of Texas Medical Branch at Galveston and Univ. Hospital Maastricht (United States)
Roel J. Erckens, Univ. of Texas Medical Branch at Galveston and Univ. Hospital Maastricht (Netherlands) (United States)
Franciscus H. M. Jongsma, Univ. Hospital Maastricht (Netherlands)
Eefke E. Smit-Kepel, Univ. of Texas Medical Branch at Galveston (United States)
Massoud Motamedi, Univ. of Texas Medical Branch at Galveston (United States)
Wayne F. March, Univ. of Texas Medical Branch at Galveston (United States)


Published in SPIE Proceedings Vol. 3608:
Biomedical Applications of Raman Spectroscopy
Michael D. Morris, Editor(s)

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