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Proceedings Paper

Time- and space-correlated single-photon counting system for fluorescence microspectroscopy of biological specimens
Author(s): Mikhail R. Ainbund; Sergei Yu. Arzhantsev; Andrey Yuri Chikishev; Klaus Kemnitz; Nikolai I. Koroteev; Aleksandr P. Shkurinov; Bulat N. Toleutaev; Evgenii A. Turbin
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Paper Abstract

It is well known that fluorescence microspectroscopy is a powerful tool in the study of biological objects. Fluorescence lifetime imaging is one of the modern and promising techniques applied in these studies. The method implies the detection of not only spectral parameters of some molecules and molecular structures but also the fluorescence lifetimes. Thus, one can easily distinguish between two fluorophores with similar spectral properties but having different fluorescence lifetimes. Additional possibilities are offered by the method for stray light rejection and detection of Raman scattering because of the difference in time-correlation of the excitation, fluorescence, and Raman photons. We suggest the application of an original position-sensitive micro-channel plate photomultiplier as a key element of a newly developed fluorescence microspectrometer designated for the study of biological objects (cells, subcellular structures and organells). We present the first results of the experiments with the test objects. The results obtained clearly show that the new system is rather promising in the studies of living cells and tissues.

Paper Details

Date Published: 5 February 1999
PDF: 6 pages
Proc. SPIE 3732, ICONO '98: Laser Spectroscopy and Optical Diagnostics: Novel Trends and Applications in Laser Chemistry, Biophysics, and Biomedicine, (5 February 1999); doi: 10.1117/12.340014
Show Author Affiliations
Mikhail R. Ainbund, ELDI (Russia)
Sergei Yu. Arzhantsev, M.V. Lomonosov Moscow State Univ. (Russia)
Andrey Yuri Chikishev, M.V. Lomonosov Moscow State Univ. (Russia)
Klaus Kemnitz, EuroPhoton GmbH (Germany)
Nikolai I. Koroteev, M.V. Lomonosov Moscow State Univ. (Russia)
Aleksandr P. Shkurinov, M.V. Lomonosov Moscow State Univ. (Russia)
Bulat N. Toleutaev, M.V. Lomonosov Moscow State Univ. (Russia)
Evgenii A. Turbin, ELDI (Russia)


Published in SPIE Proceedings Vol. 3732:
ICONO '98: Laser Spectroscopy and Optical Diagnostics: Novel Trends and Applications in Laser Chemistry, Biophysics, and Biomedicine
Andrey Yuri Chikishev; Victor N. Zadkov; Alexei M. Zheltikov, Editor(s)

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