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Proceedings Paper

Development of oxygen and pH optical sensors using phase modulation technique
Author(s): Zoe Chen-Esterlit; Jonathan W. Aylott; Raoul Kopelman
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Paper Abstract

In this paper we report the use of phase sensitive fluorometry to obtain preliminary results from micrometer- sized optical fiber oxygen sensors and a pH sensitive dye. Micrometer-sized (100 micrometers and 10 - 20 micrometers ) oxygen sensors were prepared on the distal tip of an optical fiber using a dip-coating procedure to immobilize Tris(4,7- Diphenyl-1,10-Phenanthroline)Ru(II) Chloride (Ru(dpp)) within a sol-gel matrix. These optodes exhibit excellent performance towards both gaseous and dissolved oxygen. The sensor's intensity based excitation and emission spectra, as well as phase fluorometric measurements, were collected utilizing a back-detection set-up. These micro-optodes have many desirable features for minimally invasive bio-medical applications arising from the potential of miniaturization. Carboxynaphthofluorescein solutions are shown to exhibit pH- dependent fluorescence decay times and analysis of the phase and the modulation values indicate a strong pH dependence in the physiological range, thus identifying this water-soluble dye as an attractive candidate for optical pH measurements in biomedical samples.

Paper Details

Date Published: 23 February 1999
PDF: 9 pages
Proc. SPIE 3540, Chemical, Biochemical, and Environmental Fiber Sensors X, (23 February 1999); doi: 10.1117/12.339803
Show Author Affiliations
Zoe Chen-Esterlit, Univ. of Michigan (United States)
Jonathan W. Aylott, Univ. of Michigan (United Kingdom)
Raoul Kopelman, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 3540:
Chemical, Biochemical, and Environmental Fiber Sensors X
Robert A. Lieberman, Editor(s)

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