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Proceedings Paper

Development of fluorescent fiber optic single-polymer membrane sensors for simultaneous ratiometric detection of oxygen and carbon dioxide in biological systems
Author(s): Sunil Dourado; Raoul Kopelman
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Paper Abstract

Oxygen- and carbon dioxide-sensitive fluorescent indicators are incorporated into a polymeric membrane for their detection by ratiometric fluorescence measurements. Oxygen is measured by fluorescent quenching of Ruthenium, and Platinum dyes. Carbon dioxide is monitored by tracking the change in the fluorescence emission spectrum of a pH indicator dye as the gas diffuses through the polymer membrane and interacts with the dye. The detection principle is the pH change induced in the membrane due to the influx of carbon dioxide. The indicator dye studied is HPTS. The polymer membranes are made of silicone or polystyrene.

Paper Details

Date Published: 23 February 1999
PDF: 11 pages
Proc. SPIE 3540, Chemical, Biochemical, and Environmental Fiber Sensors X, (23 February 1999); doi: 10.1117/12.339800
Show Author Affiliations
Sunil Dourado, Univ. of Michigan (United States)
Raoul Kopelman, Univ. of Michigan (United States)


Published in SPIE Proceedings Vol. 3540:
Chemical, Biochemical, and Environmental Fiber Sensors X
Robert A. Lieberman, Editor(s)

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