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Proceedings Paper

Eradication of bacterial species via photosensitization
Author(s): Paul S. Golding; L. Maddocks; Terence A. King; D. B. Drucker
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Paper Abstract

Photosensitization and inactivation efficacy of three bacterial species: Prevotella nigrescens, Staphylococcus aureus and Escherichia coli have been investigated. Samples of Staphylococcus aureus and Escherichia coli were treated with the triphenylmethane dye malachite green isothiocyanate and exposed to light from a variety of continuous and pulsed light sauces at a wavelength of approximately 630 nm. Inactivation of the Gram-positive species Staphylococcus aureus was found to increase with radiation dose, whilst Gram-negative Escherichia coli was resistant to such treatment. Samples of the pigmented species Prevotella nigrescens were found to be inactivated by exposure to light alone. The mechanism of photosensitization and inactivation of Staphylococcus aureus with malachite green isothiocyanate is addressed. The possible roles of the excited triplet state of the photosensitizer, the involvement of molecular oxygen, and the bacterial cell wall are discussed. Photosensitization may provide a way of eliminating naturally pigmented species responsible for a variety of infections, including oral diseases such as gingivitis and periodontitis.

Paper Details

Date Published: 3 February 1999
PDF: 10 pages
Proc. SPIE 3563, Photochemotherapy of Cancer and Other Diseases, (3 February 1999); doi: 10.1117/12.339135
Show Author Affiliations
Paul S. Golding, Univ. of Manchester (United Kingdom)
L. Maddocks, Univ. of Manchester (United Kingdom)
Terence A. King, Univ. of Manchester (United Kingdom)
D. B. Drucker, Univ. of Manchester (United Kingdom)

Published in SPIE Proceedings Vol. 3563:
Photochemotherapy of Cancer and Other Diseases
Benjamin Ehrenberg; Kristian Berg, Editor(s)

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