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Proceedings Paper

Clearance of protoporphyrin IX from mouse skin after topical application of 5-aminolevulinic acid and its methyl ester
Author(s): Petras Juzenas; Roar Sorensen; Vladimir Iani; Johan Moan
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Paper Abstract

The clearance of protoporphyrin IX (PpIX) from the skin of hairless BALB/c mice after topical application of 5- aminolevulinic acid (ALA) and its methyl ester (ALA-Me) was investigated. Creams containing 2 or 20% of ALA or ALA-Me were topically applied on spots of approximately 1 cm2 for 12 hours. The PpIX fluorescence was detected by the means of a Perkin Elmer LS50B luminescence spectrometer equipped with a fiber-optic probe. The emission spectrum was identical with that of cell-bound PpIX. After 12 hours application of ALA and ALA-Me similar amounts of PpIX were found. After creme removal the ALA-induced PpIX fluorescence decayed with a half-life of about 20 hours (20% ALA cream). The ALA-Me-induced PpIX was faster cleared from the skin than ALA-induced PpIX, and had a half-life of about 7 hours (20% ALA-Me cream).

Paper Details

Date Published: 3 February 1999
PDF: 6 pages
Proc. SPIE 3563, Photochemotherapy of Cancer and Other Diseases, (3 February 1999); doi: 10.1117/12.339134
Show Author Affiliations
Petras Juzenas, The Norwegian Radium Hospital and Vilnius Univ. (Norway)
Roar Sorensen, The Norwegian Radium Hospital (Norway)
Vladimir Iani, The Norwegian Radium Hospital (Norway)
Johan Moan, The Norwegian Radium Hospital (Norway)

Published in SPIE Proceedings Vol. 3563:
Photochemotherapy of Cancer and Other Diseases
Benjamin Ehrenberg; Kristian Berg, Editor(s)

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