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Proceedings Paper

PDT-induced apoptosis in bladder carcinoma cells
Author(s): Ruediger Bachor; Ella Dumbravia Reich; Klaus Kleinschmidt; Denes Repassy; Richard E. Hautmann
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Paper Abstract

Photodynamic therapy (PDT) is a highly efficient inducer of apoptosis in EY-28 bladder carcinoma cells, resulting in extensive DNA fragmentation. Bladder carcinoma cells EY-28 (Tumorbank Heidelberg, Germany) were incubated for 1 h with 1 (mu) g AamTPPn/ml or 2 (mu) g AamTPPn/ml. After incubation cells were refed with complete medium and irradiated with 0.75 J/cm2. To identify apoptotic cells, a in situ cell death detection kit POD (Boehringer Mannheim, Germany) was used. The chromatin condensation characteristic to apoptotic cells was detected by transmission electron microscopy. Using 1 (mu) g AamTPPn/ml and 2 (mu) g AamTPPn/ml (9-Acetamido-2,7,12,17- tetra-n-Porpylporphycene), respectively, and irradiation at 0.75 J/cm2, a percentage of 36.9% and 54.7%, respectively, of apoptotic cells was detected.

Paper Details

Date Published: 3 February 1999
PDF: 9 pages
Proc. SPIE 3563, Photochemotherapy of Cancer and Other Diseases, (3 February 1999); doi: 10.1117/12.339132
Show Author Affiliations
Ruediger Bachor, Univ. of Ulm (Germany)
Ella Dumbravia Reich, Univ. of Ulm (Germany)
Klaus Kleinschmidt, Univ. of Ulm (Germany)
Denes Repassy, Univ. of Budapest (Germany)
Richard E. Hautmann, Univ. of Ulm (Germany)


Published in SPIE Proceedings Vol. 3563:
Photochemotherapy of Cancer and Other Diseases
Benjamin Ehrenberg; Kristian Berg, Editor(s)

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