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Proceedings Paper

Photoimmobilization of biomolecules with a scanning near-field optical microscope: functionalization and detection at the submicrometer scale
Author(s): Claude Philipona; Patrick Lambelet; Luciana Vaccaro; Fabienne D. Marquis-Weible; Hans Sigrist
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Paper Abstract

In view of future generations of biosensors, immobilization of biomolecules with high spatial resolution onto selected materials or transducer surfaces is required. One way to attach the biomolecules is to induce covalent bonds with the substrate using a photochemical effect. High localization in the attachment is obtained when performing the photochemistry in the next field. In this paper, we present patterns of covalently bond proteins, written with near field UV activation. Observation is done in the near field as well, using near field fluorescence microscopy.

Paper Details

Date Published: 15 January 1999
PDF: 2 pages
Proc. SPIE 3570, Biomedical Sensors, Fibers, and Optical Delivery Systems, (15 January 1999); doi: 10.1117/12.336937
Show Author Affiliations
Claude Philipona, Ecole Polytechnique Federale de Lausanne (Switzerland)
Patrick Lambelet, Ecole Polytechnique Federale de Lausanne (Switzerland)
Luciana Vaccaro, Ecole Polytechnique Federale de Lausanne (Switzerland)
Fabienne D. Marquis-Weible, Ecole Polytechnique Federale de Lausanne (Switzerland)
Hans Sigrist, Ctr. Suisse d'Electronique et de Microelectronique SA (Switzerland)

Published in SPIE Proceedings Vol. 3570:
Biomedical Sensors, Fibers, and Optical Delivery Systems
Francesco Baldini; Nathan I. Croitoru; Martin Frenz; Ingemar Lundstroem; Mitsunobu Miyagi; Riccardo Pratesi; Otto S. Wolfbeis, Editor(s)

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