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Proceedings Paper

Silicon microfabricated device for a non-sheath-flow cytometer
Author(s): Martin A. Afromowitz; Ming-Chieh Huang
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Paper Abstract

We report on the design and fabrication of a low Reynolds number silicon/pyrex microfluidic device that forces particles flowing in a microchannel to a tightly-focused equilibrium position. This device can be used for optical biomedical systems such as a flow cytometer so that particles can flow in a precise position without sheath flow for precision scattering or fluorescence measurements. Devices with a range of dimensions were fabricated using photolithography and wet chemical etching techniques. Particle positions in the channel are determined by observing the direct image as well as two reflection images from the sloping, polished channel walls. We tested devices with various ratios of width to particle diameter at a range of flow rates. When scattering signals from 10 micrometers polymer beads are measured, we observe that the tight equilibrium position attained by the particles results in a much smaller standard deviation of the scattering signal than is observed in other microflow channel devices that do not focus the particles to equilibrium positions.

Paper Details

Date Published: 15 January 1999
PDF: 8 pages
Proc. SPIE 3570, Biomedical Sensors, Fibers, and Optical Delivery Systems, (15 January 1999); doi: 10.1117/12.336935
Show Author Affiliations
Martin A. Afromowitz, Univ. of Washington (United States)
Ming-Chieh Huang, Univ. of Washington (Taiwan)

Published in SPIE Proceedings Vol. 3570:
Biomedical Sensors, Fibers, and Optical Delivery Systems
Francesco Baldini; Nathan I. Croitoru; Martin Frenz; Ingemar Lundstroem; Mitsunobu Miyagi; Riccardo Pratesi; Otto S. Wolfbeis, Editor(s)

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