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Proceedings Paper

Surface plasmon resonance analysis of immobilized fibrinogen and fibrin and their interaction with thrombin and fibrinogen
Author(s): Jan E. Dyr; Marketa Jirouskova; Jitka Rysava; Ivo Tichy; Petr Tobiska; Radan Slavik; Jiri Homola; Jiri Suttnar
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Paper Abstract

The exploitation of surface plasmon resonance optical sensor for the study of the interaction of immobilized fibrinogen and fibrin monomer with soluble fibrinogen and thrombin is reported. Soluble fibrinogen was mostly reversible, the bound thrombin could be inhibited by milimolar concentration of phenylmethylsulphonyl fluoride (PMSF). At lease three sets of different thrombin binding sites were found. There was a residual fraction of thrombin bound to washed fibrin (ogin) (to about a five to ten percent of fibron monomer units) suggesting that a known naturally occurring fibrinogen variant differing in the gamma chain was the target. Surface bound fibrinogen was converted by thrombin to fibrin monomer that interacted with fibrinogen in solution. At low fibrin monomer surface density the second layer was formed that contained about the same amount of protein as the first layer, at higher fibrin monomer concentration less than one molecule of fibrinogen per molecule of fibrin monomer was captured. Starting with surface-bound fibrinogen and alternating addition of thrombin and fibrinogen a fibrin network of predetermined composition, size, and arrangement could be formed.

Paper Details

Date Published: 15 January 1999
PDF: 8 pages
Proc. SPIE 3570, Biomedical Sensors, Fibers, and Optical Delivery Systems, (15 January 1999); doi: 10.1117/12.336928
Show Author Affiliations
Jan E. Dyr, Institute of Hematology and Blood Transfusion (Czech Republic)
Marketa Jirouskova, Institute of Hematology and Blood Transfusion (Czech Republic)
Jitka Rysava, Institute of Hematology and Blood Transfusion (Czech Republic)
Ivo Tichy, Institute of Radio Engineering and Electronics (Czech Republic)
Petr Tobiska, Institute of Radio Engineering and Electronics (Czech Republic)
Radan Slavik, Institute of Radio Engineering and Electronics (Czech Republic)
Jiri Homola, Institute of Radio Engineering and Electronics (United States)
Jiri Suttnar, Institute of Hematology and Blood Transfusion (Czech Republic)


Published in SPIE Proceedings Vol. 3570:
Biomedical Sensors, Fibers, and Optical Delivery Systems
Francesco Baldini; Nathan I. Croitoru; Martin Frenz; Ingemar Lundstroem; Mitsunobu Miyagi; Riccardo Pratesi; Otto S. Wolfbeis, Editor(s)

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