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Proceedings Paper

Blood plasma coagulation studied by surface plasmon resonance
Author(s): Trine P. Vikinge; Kenny M. Hansson; Johan Benesch; Knut Johansen; Mats Ranby; Tomas L. Lindahl; Ingemar Lundstroem; Pentti Tengvall
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Paper Abstract

A surface plasmon resonance (SPR) apparatus was used to investigate blood plasma coagulation in real-time as a function of thromboplastin and heparin concentrations. The physical reason for the SPR signal observed is discussed and 3 different models are proposed. The response curves were analyzed by multivariable curve fitting followed by feature extraction. Interesting parameters of the sigmoid curves were lag time, slope and maximum response. When thromboplastin concentrations were increased, the lag-time decreased and the slope of the curve increased. A prolonged clotting time was followed mostly by increased maximum response, with exception for samples with no or very little thromboplastin added. High heparin concentrations changed the clotting kinetics. As seen from the lag-time vs. slope relation. Atomic force microscopy pictures of sensor surfaces dried after completed clotting, revealed differences in fibrin network structures as a function of thromboplastin concentration, and fiber thickness increased with lower thromboplastin concentration. The results correlate well with present common methods.

Paper Details

Date Published: 15 January 1999
PDF: 8 pages
Proc. SPIE 3570, Biomedical Sensors, Fibers, and Optical Delivery Systems, (15 January 1999); doi: 10.1117/12.336920
Show Author Affiliations
Trine P. Vikinge, Linkoeping Univ. (Sweden)
Kenny M. Hansson, Linkoeping Univ. and Univ. Hospital (Sweden)
Johan Benesch, Linkoeping Univ. (Sweden)
Knut Johansen, Linkoeping Univ. (Sweden)
Mats Ranby, Univ. Hospital (Sweden)
Tomas L. Lindahl, Univ. Hospital (Sweden)
Ingemar Lundstroem, Linkoeping Univ. (Sweden)
Pentti Tengvall, Linkoeping Univ. (Sweden)


Published in SPIE Proceedings Vol. 3570:
Biomedical Sensors, Fibers, and Optical Delivery Systems
Francesco Baldini; Nathan I. Croitoru; Martin Frenz; Ingemar Lundstroem; Mitsunobu Miyagi; Riccardo Pratesi; Otto S. Wolfbeis, Editor(s)

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