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Proceedings Paper

Rapid cleanup of bacterial DNA from samples containing aerosol contaminants
Author(s): Darrell E. Menking; Suzanne K. Kracke; Peter A. Emanuel; James J. Valdes
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Paper Abstract

Polymerase Chain Reaction (PCR) is an in vitro enzymatic, synthetic method used to amplify specific DNA sequences from organisms. Detection of DNA using gene probes allows for absolute identification not only of specific organisms, but also of genetic material in recombinant organisms. PCR is an exquisite biological method for detecting bacteria in aerosol samples. A major challenge facing detection of DNA from field samples is that they are almost sure to contain impurities, especially impurities that inhibit amplification through PCR. DNA is being extracted from air, sewage/stool samples, food, sputum, a water and sediment; however, multi- step, time consuming methods are required to isolate the DNA from the surrounding contamination. This research focuses on developing a method for rapid cleanup of DNA which combines extraction and purification of DNA while, at the same time, removing inhibitors from 'dirty samples' to produce purified, PCR-ready DNA. GeneReleaser produces PCR-ready DNA in a rapid five-minute protocol. GeneReleaser resin was able to clean up sample contain micrograms of typical aerosol and water contaminants. The advantages of using GR are that it is rapid, inexpensive, requires one-step, uses no hazardous material and produces PCR-ready DNA.

Paper Details

Date Published: 18 January 1999
PDF: 5 pages
Proc. SPIE 3533, Air Monitoring and Detection of Chemical and Biological Agents, (18 January 1999); doi: 10.1117/12.336859
Show Author Affiliations
Darrell E. Menking, U.S. Army Edgewood Research, Development, and Engineering Ctr. (United States)
Suzanne K. Kracke, Geo-Centers, Inc. (United States)
Peter A. Emanuel, U.S. Army Edgewood Research, Development, and Engineering Ctr. (United States)
James J. Valdes, U.S. Army Edgewood Research, Development, and Engineering Ctr. (United States)


Published in SPIE Proceedings Vol. 3533:
Air Monitoring and Detection of Chemical and Biological Agents
Joseph Leonelli; Mark L.G. Althouse, Editor(s)

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