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Proceedings Paper

Biological agent identification by nucleic acid base-pair analysis using surface-enhanced Raman spectroscopy
Author(s): Stuart Farquharson; Wayne W. Smith; Susan Elliott; Jay F. Sperry
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Paper Abstract

Recently, a number of analytical methods have been successfully developed which use nucleic acid sequencing to identify biological warfare agents. However, the effectiveness of these methods, towards the safety and protection of US Armed Forces and their allies are limited by the period required to enumerate the nucleic acid through polymerase chain reactions or culture growth to produce sufficient quantities for analysis. To overcome this limitation, we have been investigating the ability of surface-enhanced Raman spectroscopy to detect nucleic acids with sufficient sensitivity and selectivity to eliminate the need for enumeration. The design of a small volume electrolytic sample cell will be presented along with analysis of the nucleic acid bases and preliminary analysis of model bacteria.

Paper Details

Date Published: 18 January 1999
PDF: 8 pages
Proc. SPIE 3533, Air Monitoring and Detection of Chemical and Biological Agents, (18 January 1999); doi: 10.1117/12.336858
Show Author Affiliations
Stuart Farquharson, Advanced Fuel Research, Inc. (United States)
Wayne W. Smith, Advanced Fuel Research, Inc. (United States)
Susan Elliott, Univ. of Rhode Island (United States)
Jay F. Sperry, Univ. of Rhode Island (United States)


Published in SPIE Proceedings Vol. 3533:
Air Monitoring and Detection of Chemical and Biological Agents
Joseph Leonelli; Mark L.G. Althouse, Editor(s)

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