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Proceedings Paper

Electron and scanning probe microscopy study of S-layers of plague microbes
Author(s): Nikolai P. Konnov; Vil B. Baiburin; Ivan A. Djatlov; Oksana A. Antonova; Uryi P. Volkov
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Paper Abstract

Microscopy investigations of S-layers of plague microbes have been carried out. A protein forming S-layer was isolated, purified and its biochemical properties were studied. Images of plague cells with and without S-layer and images of isolated S-layer protein arrays on solid supports were obtained by transmission electron microscopy. An isolated protein forming the layer is self-assembled into crystalline structure with hexagonal pores are of regular size about 4 - 8 nm, which was observed by transmission electron micrographs. The STM images of plague microbes with S-layer and without one and the STM and AFM images of isolated S-layer protein arrays on mica surface were obtained.

Paper Details

Date Published: 19 January 1999
PDF: 5 pages
Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); doi: 10.1117/12.336835
Show Author Affiliations
Nikolai P. Konnov, Russian Research Anti-Plague Institute Microbe (Russia)
Vil B. Baiburin, Saratov Technical Univ. (Russia)
Ivan A. Djatlov, Russian Research Anti-Plague Institute Microbe (Russia)
Oksana A. Antonova, Russian Research Anti-Plague Institute Microbe (Russia)
Uryi P. Volkov, Saratov Technical Univ. (Russia)


Published in SPIE Proceedings Vol. 3568:
Optical Biopsies and Microscopic Techniques III
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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