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Proceedings Paper

Phagocytosis: studies by optical tweezers and time-resolved microspectrofluorometry
Author(s): Herbert Schneckenburger; Reinhard Sailer; Anita Hendinger; Michael H. Gschwend; Manfred Bauer; Wolfgang S. L. Strauss
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Paper Abstract

Cellular uptake of transparent Latex particles by J774A.1 mouse macrophages has been studied: First, single beads were kept within an optical light trap and located in close vicinity to individual cells. Uptake of the beads was visualized, and intrinsic fluorescence was detected in the spectral range of 420 - 530 nm. Second, time-gated fluorescence spectra of single cells were recorded at pre- selected times during one hour after cellular uptake. A rapid increase of autofluorescence and a subsequent decrease to the level of control cells within about 10 min. was measured within a time gate of 0 - 5 ns after the exciting laser pulses, and attributed to the 'free' coenzyme NAD(P)H. In contrast, fluorescence increase of NAD(P)H bound to proteins (measured within time gates of 5 - 10 ns or 10 - 15 ns) was less pronounced, and the subsequent decrease occurred within a longer period (about one hour).

Paper Details

Date Published: 19 January 1999
PDF: 6 pages
Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); doi: 10.1117/12.336829
Show Author Affiliations
Herbert Schneckenburger, Univ Ulm and Fachhochschule Aalen (Germany)
Reinhard Sailer, Univ. Ulm (Germany)
Anita Hendinger, Fachhochschule Aalen (Germany)
Michael H. Gschwend, Univ. Ulm (Germany)
Manfred Bauer, Fachhochschule Aalen (Germany)
Wolfgang S. L. Strauss, Univ. Ulm (Germany)


Published in SPIE Proceedings Vol. 3568:
Optical Biopsies and Microscopic Techniques III
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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