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Proceedings Paper

Selective detection of mitochondrial malfunction in situ by energy transfer spectroscopy
Author(s): Herbert Schneckenburger; Michael H. Gschwend; Reinhard Sailer; Wolfgang S. L. Strauss; Lars Schoch; Alexander Schuh; Karl Stock; Rudolf W. Steiner; Peter Zipfl
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Paper Abstract

To establish optical in situ detection of mitochondrial malfunction, non-radiative energy transfer from the coenzyme NADH to the mitochondrial marker rhodamine 123 (R123) was examined. Dual excitation of R123 via energy transfer from excited NADH molecules as well as by direct absorption of light results in two fluorescence signals whose ratio is a measure of mitochondrial NADH. These signals are detected simultaneously using a time-gated (nanosecond) technique for energy transfer measurements and a frequency selective technique for direct excitation and fluorescence monitoring of R123. Optical and electronic components of the experimental setup are described and compared with a previously established microscopic system.

Paper Details

Date Published: 19 January 1999
PDF: 6 pages
Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); doi: 10.1117/12.336827
Show Author Affiliations
Herbert Schneckenburger, Univ. Ulm and Fachhochschule Aalen (Germany)
Michael H. Gschwend, Univ. Ulm (Germany)
Reinhard Sailer, Univ. Ulm (Germany)
Wolfgang S. L. Strauss, Univ. Ulm (Germany)
Lars Schoch, Fachhochschule Aalen (Germany)
Alexander Schuh, Fachhochschule Aalen (Germany)
Karl Stock, Univ. Ulm and Fachhochschule Aalen (Germany)
Rudolf W. Steiner, Univ. Ulm (Germany)
Peter Zipfl, Fachhochschule Aalen (Germany)

Published in SPIE Proceedings Vol. 3568:
Optical Biopsies and Microscopic Techniques III
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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