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Proceedings Paper

FISH analysis of the arrangement of chromosomes in interphase nuclei using telomeric, centromeric, and DNA painting probes
Author(s): Shamci Monajembashi; Eberhard Schmitt; Heike Dittmar; Karl-Otto Greulich
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Paper Abstract

Fluorescence in situ hybridization is used to study the arrangement of chromosomes in interphase nuclei of unsynchronized human lymphocytes. DNA probes specific for telomeric DNA, centromeric (alpha) -satellite DNA and whole chromosomes 2, 7, 9 and X are employed. It is demonstrated that the shape of the chromosome territories is variable in cycling cells, for example, close to the metaphase chromosome homologues are arranged pairwise. Furthermore, the relative arrangement of chromosome homologues to each other is not spatially defined. Also, the relative orientation of centromeres and telomeres within a chromosome domain is variable.

Paper Details

Date Published: 19 January 1999
PDF: 12 pages
Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); doi: 10.1117/12.336825
Show Author Affiliations
Shamci Monajembashi, Institut fuer Molekulare Biotechnologie e.V. (Germany)
Eberhard Schmitt, Institut fuer Molekulare Biotechnologie e.V. (Germany)
Heike Dittmar, Institut fuer Molekulare Biotechnologie e.V. (Germany)
Karl-Otto Greulich, Institut fuer Molekulare Biotechnologie e.V. (Germany)


Published in SPIE Proceedings Vol. 3568:
Optical Biopsies and Microscopic Techniques III
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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