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Proceedings Paper

Estimation of intracellular pH in argon laser scanning confocal microscopes with a laser line of 488 nm
Author(s): Jan Slavik; Edvard Lanz
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Paper Abstract

The reliable and widely used pH-sensitive indicator BCECF (2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein) can also be employed in a classical confocal laser scanning microscopes using 488 nm argon laser excitation line. The intracellular pH maps based on the fluorescence emission ratio tally with those obtained by other techniques.

Paper Details

Date Published: 19 January 1999
PDF: 5 pages
Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); doi: 10.1117/12.336822
Show Author Affiliations
Jan Slavik, Institute of Physiology (Czech Republic)
Edvard Lanz, Institute of Physiology (Czech Republic)


Published in SPIE Proceedings Vol. 3568:
Optical Biopsies and Microscopic Techniques III
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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