Share Email Print
cover

Proceedings Paper

Photoinduced effect of hypericin on collagen and tissues with high collagen content
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

The photoinduced effects of hypericin, a polycyclic quinone, on collagen has been investigated. It was found that after laser irradiation at both 532 nm and 337 nm, the spectral form of triple helix structure collagen fluorescence, changed to a spectral profile bearing resemblance to that of its polypeptide single chain counterpart, gelatin, or heated collagen. The effect of Chlorin e6 on collagen was also investigated and proved to be dissimilar to that of hypericin and not indicative of profound structural alterations. Second Harmonic Generation (SHG) of 1064 nm- nanosecond laser radiation in collagen was studied. While it was very efficient for pure collagen, the signal intensity was found to diminish by at least an order of magnitude after hypericin photosensitization or heating. The above noted fluorescence spectra form alteration was also observed in a smaller scale in collagen rich chicken tissue (tendon). Non sensitized chicken tendon tissue exhibited very efficient SHG, unlike skin and artery samples.

Paper Details

Date Published: 4 January 1999
PDF: 7 pages
Proc. SPIE 3565, Thermal Therapy, Laser Welding, and Tissue Interaction, (4 January 1999); doi: 10.1117/12.335800
Show Author Affiliations
Dido M. Yova, National Technical Univ. of Athens (Greece)
Theodossis Theodossiou, National Technical Univ. of Athens (United Kingdom)
Vladimir A. Hovhannisyan, Yerevan State Univ. (Armenia)


Published in SPIE Proceedings Vol. 3565:
Thermal Therapy, Laser Welding, and Tissue Interaction
Stephen G. Bown; Guy P. Delacretaz; Guilhem Godlewski; Gerhard J. Mueller; Roberto Pini; Hans-Dieter Reidenbach; Rudolf W. Steiner; Lars Othar Svaasand; Karl-Goran Tranberg, Editor(s)

© SPIE. Terms of Use
Back to Top